Separation of steroids on "High Definition" phase

Application Number
403810
Method
TLC
Results
SIL HD UV254

Title: SIL HD UV254

Competitor M

Title: Competitor M


Substances
hydrocortisone; cortexolone; methyltestosterone
Product(s)
PhaseREFWebshop

SIL HD

809223

Matrix
-
Sample(s)

0.2 % in acetone

Conditions
  • Applied volume: 1 µL
  • Eluent: chloroform - methanol (97:3, v/v)
  • Migration: 6 cm in 15 min
Detection
Treatment with potassium permanganate staining solutionand subsequent drying at 120 °C for 10 min.
Note
The excellent staining property of SIL HD provides a better visibility.
Author
TLC department
Source
MACHEREY-NAGEL, Germany 2018
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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