Separation of steroids on "High Definition" phase

Separation of steroids on "High Definition" phase

Separation of steroids on "High Definition" phase

Application Number
403810
Method
TLC
Results
SIL HD UV254

Title: SIL HD UV254

Competitor M

Title: Competitor M


Substances
hydrocortisone; cortexolone; methyltestosterone
Product(s)
PhaseREFWebshop

SIL HD

809223

Matrix
-
Sample(s)

0.2 % in acetone

Conditions
  • Applied volume: 1 µL
  • Eluent: chloroform - methanol (97:3, v/v)
  • Migration: 6 cm in 15 min
Detection
Treatment with potassium permanganate staining solutionand subsequent drying at 120 °C for 10 min.
Note
The excellent staining property of SIL HD provides a better visibility.
Author
TLC department
Source
MACHEREY-NAGEL, Germany 2018
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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