Separation of aflatoxins on "High Definition" phase (HPTLC)

Application Number
403750
Method
TLC
Results
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Legend: Nano-SIL HD

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Legend: Competitor M

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Legend: Rf values

Substances
aflatoxin B1 (AFB1); aflatoxin B2 (AFB2); aflatoxin G1 (AFG1); aflatoxin G2 (AFG2)
Product(s)
PhaseREFWebshop

Nano-SIL HD

811213

Matrix
-
Sample(s)
  • AFB1, AFG1: 2 µg/mL in acetonitrile
  • AFB2, AFG2: 0.5 µg/mL in acetonitrile
Conditions
  • Applied volume: 1 µL
  • Eluent: chloroform - acetone (90:10, v/v)
  • Migration: 5 cm in appr. 15 min
Detection
TLC/HPTLC scanner UV 366 nm
Note
For a fast and cost efficient separation of aflatoxins we recommend Nano-SIL HD. Best results can be achieved with UV light of wavelength 366 nm.
Author
TLC department
Source
MACHEREY-NAGEL, Germany 2018
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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