Comparison of ALUGRAM Xtra Sil G UV254 and ALUGRAM Xtra Nano-Sil G UV254 for the separation of dexpanthenol from wound and healing ointment

Comparison of ALUGRAM Xtra Sil G UV254 and ALUGRAM Xtra Nano-Sil G UV254 for the separation of dexpanthenol from wound and healing ointment

Comparison of ALUGRAM Xtra Sil G UV254 and ALUGRAM Xtra Nano-Sil G UV254 for the separation of dexpanthenol from wound and healing ointment

Application Number
403630
Method
TLC
Results

Legend:
1 - aqueous extract 2 µL 2 - standard 1 µL 3 - methanolic extract 2 µL

Substances
dexpanthenol; 3-aminopropanol
Product(s)
PhaseREFWebshop

Xtra Nano-SIL G UV254

818343

Xtra SIL G UV254

818333

Matrix
ointment
Sample(s)

250 mg wound and healing ointment ( 5 % dexpanthenol) are taken up in 5 mL water or methanol and sonicated for 5 min

Conditions

Applied volume: 2 µL aqueous or methanolic extract Eluent: 1-butanol - methanol - ammonia (5.5:2.5:2.0, v/v/v) Migration time: 20 min

Detection
after developing and drying, the plate is immersed in 0.5 % ethanolic ninhydrine solution and dried at 125 °C
Note
Due to the use of nanoparticles on ALUGRAM Xtra Nano-SIL G UV254 the dexpanthenol spot can seen much better as on ALUGRAM Xtra SIL G UV254.
Author
TLC department
Source
MACHEREY-NAGEL, Germany 2011
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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