Amino acids
Amino acids
Amino acids
Amino acids
Amino acids
- Application Number
- 400210
- Method
- TLC
- Results
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Legend:
1. L-Met-2-naphthylamide 2. DL-Met-2-naphthylamide 3. D-Leu-2-naphthylamide 4. DL-Leu-2-naphthylamide m1 Mixture of component 3 and 4 5. L-Leu-p-nitroanilide 6. D-Leu-p-nitroanilide m2 Mixture of component 5 and 6 7. L-Ala-p-nitroanilide 8. D-Ala-p-nitroanilide- Substances
- met-naphthylamide; leu-naphthylamide; leu-nitroanilide; ala-nitroanilide
- Product(s)
Phase REF Webshop Nano-SIL C18-50 UV254
811064
- Matrix
- -
- Sample(s)
TLC of 2-naphthylamide and p-nitroanilide derivatives of amino acids
- Conditions
Eluent: 2.5 g NaCl + 26 g urea in water, dissolved to 100 mL; 20 mL acetonitrile; 0.15 M b-cyclodextrin
Sample volume: 0.2-0.5 µL of a 1 mg/mL sample in water / acetonitrile (40/60) and cyclohexane
- Detection
- UV, 254 nm
- Note
- Author
- Lepri L.
- Source
- J. Planar Chromatog. 4 (1991) 338-340
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