Determination of pyrrolizidine alkaloids - Solid phase extraction using very strong mixed mode cation exchanger
Determination of pyrrolizidine alkaloids - Solid phase extraction using very strong mixed mode cation exchanger
Determination of pyrrolizidine alkaloids - Solid phase extraction using very strong mixed mode cation exchanger
Determination of pyrrolizidine alkaloids - Solid phase extraction using very strong mixed mode cation exchanger
Determination of pyrrolizidine alkaloids - Solid phase extraction using very strong mixed mode cation exchanger
- Application Number
- 306630
- Method
- SPE
- Results
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- Substances
- monocrotaline (Mc); erucifoline (Er); monocrotaline-N-oxide (McN); jacobine (Jb); intermedine (Im); erucifoline-N-oxide (ErN); europine (Eu); lycopsamine (La); jacobine-N-oxide (JbN); europine-N-oxide (EuN); intermedine-N-oxide (ImN); lycopsamine-N-oxide (LaN); retrorsine (Re); trichodesmine (Td); retrorsine-N-oxide (ReN); seneciphylline (Sp); heliotrine (Hn); seneciphylline-N-oxide (SpN); heliotrine-N-oxide (HnN); senecionine (Sc); senecivernine (Sv); senecionine-N-oxide (ScN); senecivernine-N-oxide (SvN); echimidine (Em); echimidine-N-oxide (EmN); senkirkine (Sk); lasiocarpine (Lc); lasiocarpine-N-oxide (LcN)
- Product(s)
Phase REF Webshop CHROMABOND HR-XC
730952
- Matrix
- aqueous, methanolic
- Sample(s)
The following analysis were performed with standard solutions.
- Conditions
Conditioning: 5 mL methanol, 5 mL sulphuric acid (0.05 M)
Sample application: 10 mL standard solution in aqueous or methanolic sulphuric acid (0.05 M) with a flow rate of 3 mL/min
Washing: 6 mL water with a flow rate of 3 mL/min or 6 mL methanol with a flow rate of 3 mL/min
Drying: 5-10 min with vacuum
Elution: 2 x 5 mL 2.5 % ammonia in methanol
Eluent exchange: Evaporate eluate to a volume of 0.5 mL at 40 °C under a stream of nitrogen and fill up to 1.0 mL with water - methanol (95:5, v/v).
- Detection
- -
- Subsequent analysis
- LC-MS/MS, using core-shell phase, see MN Appl. No. 127480 or using full porous silica, see MN Appl. No. 127490.
- Note
- Solid phase extraction using RP phases see MN Appl. No. 306620. More information about the determination of pyrrolizidine alkaloids can be found in the selected MN application note 03/2018!
- Author
- Application department
- Source
- MACHEREY-NAGEL, Germany 2018
- Disclaimer
- Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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