Fractions of acidic, neutral and basic analytes from serum on CHROMABOND HR-XC
Fractions of acidic, neutral and basic analytes from serum on CHROMABOND HR-XC
Fractions of acidic, neutral and basic analytes from serum on CHROMABOND HR-XC
Fractions of acidic, neutral and basic analytes from serum on CHROMABOND HR-XC
Fractions of acidic, neutral and basic analytes from serum on CHROMABOND HR-XC
- Application Number
- 304780
- Method
- SPE
- Results
.gif "Recovery rates of fraction A (acidic and neutral analytes)")
Title: Recovery rates of fraction A (acidic and neutral analytes)
.gif "Recovery rates of fraction B (basic analytes)")
Title: Recovery rates of fraction B (basic analytes)
- Substances
- suprofen; naproxen; tolmetin; phenobarbital; indomethacin; hexobarbital; doxepin; imipramine; amitriptyline; trimipramine
- Product(s)
Phase REF Webshop CHROMABOND HR-XC
730952
- Matrix
- serum
- Sample(s)
1 mL spiked serum (1 µg/mL) + 200 µL 2 % H3PO4
- Conditions
Column conditioning: 5 mL methanol, then 5 mL water (Do not let run the column dry!)
Sample aspiration: The prepared sample is passed through the column by vacuum or pressure.
Washing: With 2 mL 0.1 N HCl impurities are removed.
Drying: With nitrogen or air
Elution: Fraction A (acidic and neutral analytes) is eluted with 2.5 mL methanol Fraction B (basic analytes) with 5.0 mL methanol / NH3 (90:10, v/v)
Evaporation and reconstitution with 1 mL of mobile phase from subsequent HPLC
- Detection
- -
- Subsequent analysis
- Fraction A: HPLC determination on EC 125/4 NUCLEODUR C18 Gravity, 5 µm (REF 760100.40) in reference to Appl. No. 122230 Fraction B: HPLC determination on EC 125/4 NUCLEODUR C8 Gravity, 5 µm (REF 760751.40) in reference to Appl. No. 118520
- Note
- Author
- SPE department
- Source
- MACHEREY-NAGEL (2008)
- Disclaimer
- Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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