Extraction of THC and 11-Nor-Δ9-THC-carboxylic acid from blood, plasma or serum
Extraction of THC and 11-Nor-Δ9-THC-carboxylic acid from blood, plasma or serum
Extraction of THC and 11-Nor-Δ9-THC-carboxylic acid from blood, plasma or serum
Extraction of THC and 11-Nor-Δ9-THC-carboxylic acid from blood, plasma or serum
Extraction of THC and 11-Nor-Δ9-THC-carboxylic acid from blood, plasma or serum
- Application Number
- 303890
- Method
- SPE
- Results
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Title: Recovered concentrations of spiked samples
- Substances
- THC; THC-COOH; 11-OH-THC
- Product(s)
Phase REF Webshop CHROMABOND Drug II
730680
- Matrix
- serum
- Sample(s)
1 mL serum and 5 mL phosphate buffer (pH 7, 0.067 M) are vortexed and subsequently centrifuged.
- Conditions
Column conditioning: 3 mL methanol, followed by 3 mL phosphate buffer (pH 7, 0.067 M)
Sample application: Aspirate slowly the sample through the column without vacuum (1-2 mL per min).
Column washing: Elute interferences with 3 mL acetate buffer (pH 4, 0.1 M) - methanol (1:1, v/v), followed by 3 mL water - methanol (1:1, v/v).
Drying: Dry the sample 5 min in vacuum.
Elution: Elute THC and metabolites with 1.5 mL acetone - glacial acetic acid (99:1, v/v).
- Detection
- -
- Subsequent analysis
- Derivatisation with MSTFA (REF 701270) GC-MS on OPTIMA 5 MS, 25 m, 0.20 µm, 0.20 mm ID (REF 726210.25): Temp.: 150 °C hold for 1 min, 9 °C/min --> 260 °C, 30 °C/min --> 300 °C, hold 5 min at 300 °C
- Note
- Author
- Köhler, H.
- Source
- Universitätsklinikum Münster, Inst. für Rechtsmedizin, Forensische Toxikologie, Germany 2005
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