Analysis of PFAS in aqueous samples by SPE and LC-MS/MS according to EPA Method 1633

Analysis of PFAS in aqueous samples by SPE and LC-MS/MS according to EPA Method 1633

Analysis of PFAS in aqueous samples by SPE and LC-MS/MS according to EPA Method 1633

Application Number
129620
Method
HPLC
Results
Substances
PFAS, see recovery rates
Product(s)
PhaseREFWebshop

NUCLEODUR® Phenyl-Hexyl

760576.20

NUCLEODUR® Phenyl-Hexyl

760573.20

Matrix
n aqueous, solid (soil, biosolids, sediment) and tissue samples
Sample(s)

Sample preparation routine is found in application database entry 307040.

Conditions

DELAY Column EC 50/2 NUCLEODUR® Phenyl-Hexyl (REF 760573.20) Column EC 100/2 NUCLEODUR® Phenyl-Hexyl, 3 µm (REF 760576.20) Eluent A 5 mM ammonium acetate in water Eluent B 5 mM ammonium acetate in methanol Gradient Hold 40 % B for 1 min, in 8 min from 40 % B to 95 % B,hold 95 % B for 3 min, in 0.1 min to 40 % B, hold 40 % B for 2.9 min Flow rate 0.3 mL/min Temperature 40 °C Injection volume 2 µL

Detection
LC-MS/MS
Note
For detailed sample preparation routine, please visit entry 307040. For further information, please download the application note „Analysis of PFAS in aqueous samples by SPE and LC-MS/MS according to EPA Method 1633“: https://www.mn-net.com/chromatography-downloads
Author
Application Department
Source
Macherey-Nagel, 2025
Disclaimer
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