Selectivity improvement by changing the organic modifier: Separation of sulfonamides

Selectivity improvement by changing the organic modifier: Separation of sulfonamides

Selectivity improvement by changing the organic modifier: Separation of sulfonamides

Application Number
128030
Method
HPLC
Results

Legend:
1 - sulfadiazine 2 - sulfachloropyridazine 3 - sulfadimidine 4 - sulfamerazine 5 - sulfamethoxazole

Substances
sulfadiazine; sulfachloropyridazine; sulfadimidine; sulfamerazine; sulfamethoxazole
Product(s)
PhaseREFWebshop

NUCLEODUR π²

760622.30

Matrix
Sample(s)
Conditions

Eluent systems (isocratic): A - methanol/water, 0.1 % TFA (35/65, v/v) B - acetonitrile/water, 0.1 % TFA (31/69, v/v)*

Flow rate: 0.56 mL/min Temperature: 25 °C Injection volume: 0.5 µL

  • optimized ratio for C18 phasel
Detection
UV, 280 nm
Note
Author
Innovation center
Source
MACHEREY-NAGEL, Germany 2017
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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