Selectivity improvement by changing the organic modifier: Separation of steroids

Selectivity improvement by changing the organic modifier: Separation of steroids

Selectivity improvement by changing the organic modifier: Separation of steroids

Application Number
128020
Method
HPLC
Results

Legend:
1 - estriol 2 - hydrocortisone 3 - cortisone 4 - cortisone acetate 5 - estrone

Substances
estriol; hydrocortisone; cortisone; cortisone acetate; estrone
Product(s)
PhaseREFWebshop

NUCLEODUR π²

760622.30

Matrix
Sample(s)
Conditions

Eluent systems (isocratic): A - methanol/water (75/25, v/v) B - acetonitrile/water (59/41, v/v)*

Flow rate: 0.56 mL/min Temperature: 25 °C Injection volume: 0.5 µL

  • optimized ratio for C18 phase
Detection
UV, 230 nm
Note
Author
Innovation center
Source
MACHEREY-NAGEL, Germany 2017
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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