Comparison of selectivity for sulfonamide antibiotics

Comparison of selectivity for sulfonamide antibiotics

Comparison of selectivity for sulfonamide antibiotics

Application Number
127920
Method
HPLC
Results

Legend:
1 - sulfathiazole 2 - sulfadiazine 3 - sulfachloropyridazine 4 - sulfamerazine 5 - sulfadimidine 6 - sulfamethoxazole 7 - sulfadimethoxine 8 - sulfaquinoxaline

Substances
sulfathiazole; sulfadiazine; sulfachloropyridazine; sulfamerazine; sulfadimidine; sulfamethoxazole; sulfadimethoxine; sulfaquinoxaline
Product(s)
PhaseREFWebshop

NUCLEODUR π²

760622.30

Matrix
Sample(s)
Conditions

Eluent A: 0.1% TFA in water Eluent B: 0.1% TFA in methanol Gradient: 20% B for 2 min, 20 - 60% B in 2 min, 60% B for 10 min Flow rate: 0.56 mL/min Temperature: 30 °C Injection volume: 1.0 µL

Detection
UV, 280 nm
Note
Compared to other biphenyl columns NUCLEODUR π² provides baseline separation of different sulfonamide antibiotics.
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2016
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