The P. putida strains were inoculated with a respective overnight culture (1:100) and incubated in 300 mL flasks containing 50 mL LB medium supplemented with tetracycline (25 µg/mL) and with 2 % XAD 16 resin for 1 - 2 h at 30 °C with shaking. Myxothiazol production was induced by the addition of toluic acid (5 mM); the culture was transferred to 16 °C and incubated for 2 - 3 days. The cells and resin were harvested by centrifugation and extracted with acetone and methanol. The extracts were evaporated and resuspended in 1 mL methanol; 5 µL of the extracts was analyzed by LC-MS.

Eluent A: water with 0.1% formic acid Eluent B: acetonitrile with 0.1 % formic acid Gradient: 5 % B for 2 min, 5-95 % B in 30 min, 95 % B for 4 min Flow rate: 0.4 mL/min Injection volume: 5 µL