Comparing of selectivity for nucleobases

Comparing of selectivity for nucleobases

Comparing of selectivity for nucleobases

Application Number
127270
Method
HPLC
Results

Legend:
1 - cytosine 2 - adenine 3 - uracil 4 - guanine 5 - thymine

Substances
cytosine; adenine; uracil; guanine; thymine
Product(s)
PhaseREFWebshop

NUCLEODUR C18 Gravity-SB

760618.46

NUCLEODUR C18 Gravity

760103.46

NUCLEODUR C18 Pyramid

760203.46

Matrix
-
Sample(s)

1 mg/mL each, solved in eluent

Conditions
  • Eluent: 25 mmol/L KH2PO4, pH 3 - methanol (95:5, v/v)
  • Flow rate: 1.0 mL/min
  • Temperature: 20 °C
  • Injection volume: 2.5 µL
Detection
UV, 220 nm
Note
NUCLEODUR C18 Gravity-SB provides a better resolution of early eluting compounds
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2015
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
Dateien
Lädt...