Use of 2D-chromatography in the separation of explosives
Use of 2D-chromatography in the separation of explosives
Use of 2D-chromatography in the separation of explosives
- Application Number
- 127210
- Method
- HPLC
- Results
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Legend:
1 - 2,4,6-trinitrophenylmethylnitramine 2 - diethylene glycol dinitrate 3 - hexahydro-1,3,5-trinitro-1,3,5-triazine 4 - picric acid 5 - ethylene glycol dinitrate 6 - 1,3,5-trinitrobenzene 7 - 1,3-dinitrobenzene 8 - 3,5-dinitroaniline 9 - nitrobenzene 10 - nitroglycerine 11 - 2,4,6-trinitrotoluene 12 - octogen 13 - 2-amino-4,6-dinitrotoluene 14 - 4-amino-2,6-dinitrotoluene 15 - 2,4-dinitrotoluene 16 - 2,6-dinitrotoluene 17 - 2-nitrotoluene 18 - 4-nitrotoluene 19 - 3-nitrotoulene 20 - pentaerythritol tetranitrate 21 - diphenylamine 22 - hexanitrodiphenylamine- Substances
- 2,4,6-trinitrophenylmethylnitramine; diethylene glycol dinitrate; hexahydro-1,3,5-trinitro-1,3,5-triazine; picric acid; ethylene glycol dinitrate; 1,3,5-trinitrobenzene; 1,3-dinitrobenzene; 3,5-dinitroaniline; nitrobenzene; nitroglycerine; 2,4,6-trinitrotoluene; octogen; 2-amino-4,6-dinitrotoluene; 4-amino-2,6-dinitrotoluene; 2,4-dinitrotoluene; 2,6-dinitrotoluene; 2-nitrotoluene; 4-nitrotoluene; 3-nitrotoulene; pentaerythritol tetranitrate; diphenylamine; hexanitrodiphenylamine
- Product(s)
Phase REF Webshop NUCLEOSHELL RP 18
763136.20
NUCLEOSHELL RP 18plus
763232.20
NUCLEOSHELL PFP
763532.20
NUCLEOSHELL Phenyl-Hexyl
763732.20
- Matrix
- Sample(s)
mix of compounds each 1 µg/mL water - methanol (1:4, v/v)
For an extraction from well water we recommend SPE appl. no. 305920
- Conditions
Binary Gradient System Eluent A: water - methanol - 25 mmol/L ammonium acetate pH 4.0 (75:15:10, v/v/v) Eluent B: methanol - 25 mmol/L ammonium acetate pH 4.0 (90:10, v/v) Gradient: 0 % B to 100 % B in 40 min, hold for 10 min Flow rate: 0.300 mL/min
Ternary Gradient System (alternative eluent system achieving same chomatogramms) Eluent A: water Eluent B: methanol Eluent C: 25 mmol/L ammonium acetate pH 4.0 Total Flow: 0.300 mL/min Gradient: 15 % B to 80 % B in 40 min, hold for 10 min at a flow rate of 0.27 mL/min, Eluent C is added constant at a flow rate of 0.03 mL/min over the hole analysis
Temperature: 40 °C Injection volume: 25 µL
- Detection
- UV, 210 nm, 230 nm, 254 nm, 360 nm
- Note
- The chromatographic results illustrate that each of the column combinations has advantages in the separation of different classes of explosives due to the combined selectivities. Especially, a separation of the 19 analytes described by EPA 8330B in a single run is made possible by the combination of the columns NUCLEOSHELL RP 18 and PFP. If the respective explosives categories are supplemented by other analytes such as degradation products, the results shown are good starting conditions for further optimizations. More information about the analysis of trace residues of explosive materials following the guidelines set out in USEPA method 8330B and DIN EN ISO 22478 can be found in the selected MN application note 02/2017!
- Author
- Application department
- Source
- MACHEREY-NAGEL, Germany 2014
- Disclaimer
- Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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