Novel expression hosts for complex secondary metabolite megasynthetases: Production of myxochromide in the thermophilic isolate Corallococcus macrosporus GT-2

Application Number: 126290
Method: HPLC
Results: Legend:
Chromatograms: 1 - extract of C. macrosporus GT-2 2 - 4 - extracts of mutant strains containing myxochromide gene cluster integrated into the chromosome of GT-2 Inset panel: UV spectrum of myxochromide S2 at 400 nm
Substances: myxochromide S1; myxochromide S2; myxochromide S3
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NUCLEODUR C18 ec

760051.20

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761100.30
Matrix: bacterium
Sample(s): For production of myxochromide and for sample preparation of culture extracts see the cited literature.
Conditions: Eluent A: water + 0.1 % formic acid Eluent B: acetonitrile + 0.1 % formic acid Gradient: 60 % B for 1 min, 60-95 % B in 6 min, then 95 % B for remaining time Injection volume: 5 µL
Detection: UV, 400 nm
Note
Author: Perlova, O. et al.
Source: Microbial Cell Factories 2009, 8: 1
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NUCLEODUR C18 ec	760051.20	Go to product details
	761100.30