Separation of β-lactam antibiotics on NUCLEOSHELL Phenyl-Hexyl with MS detection

Separation of β-lactam antibiotics on NUCLEOSHELL Phenyl-Hexyl with MS detection

Separation of β-lactam antibiotics on NUCLEOSHELL Phenyl-Hexyl with MS detection

Application Number
126030
Method
HPLC
Results
Substances
cefoxitin; cloxacillin; nafcillin; dicloxacillin
Product(s)
PhaseREFWebshop

NUCLEOSHELL Phenyl-Hexyl

763732.20

Matrix
Sample(s)

mix of compounds solved in eluent

Conditions

Eluent: methanol - 10 mmol/L ammonium formate, pH 3.0 (50:50, v/v) Flow rate: 0.45 mL/min Temperature: 40 °C Injection volume: 1.0 µL

Detection
MS, ESI negative Ion spray voltage: -4500 V Curtain gas: 20 psi Temperature: 550 °C
Note
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2012
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