Eluent A: methanol
Eluent B: water with 0.1 % formic acid
Gradient: 67.5 % A for 12.5 min, to 90 % A in 12.5 min
Flow rate: 0.56 mL/min
Temperature: 25 °C
Injection volume: 5.0 µL
Detection
UV, 254 nm
Note
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2011
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