Comparison of polar embedded phases: Separation of pyridine and phenol under identical conditions
- Application Number
- 124711
- Method
- HPLC
- Results
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Legend:
1 - pyridine 2 - phenol- Substances
- pyridine; phenol
- Product(s)
Phase REF Webshop NUCLEODUR PolarTec
760488.30
competitor phase with similar modification
on request
- Matrix
- Sample(s)
each compound 1 mg/mL in eluent
- Conditions
Eluent : acetonitrile - water (50:50, v/v) Flow rate: 0.56 mL/min Temperature: 25 °C Injection volume: 1.0 µL
- Detection
- UV, 254 nm
- Note
- The separation of pyridine and phenol is used to determine the base deactivation. On a good base deactivated phase pyridine is eluted well ahead of phenol. Furthermore the pyridine peak has to be symmetric. NUCLEODUR PolarTec shows a high resolution between pyridine and phenol, and the pyridine peak is symmetric. This indicates a very good base deactivation.
- Author
- HPLC department
- Source
- MACHEREY-NAGEL, Germany 2011
- Disclaimer
- Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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