Comparison of polar embedded phases: Separation of pyridine and phenol under identical conditions

Comparison of polar embedded phases: Separation of pyridine and phenol under identical conditions

Comparison of polar embedded phases: Separation of pyridine and phenol under identical conditions

Application Number
124711
Method
HPLC
Results

Legend:
1 - pyridine 2 - phenol

Substances
pyridine; phenol
Product(s)
PhaseREFWebshop

NUCLEODUR PolarTec

760488.30

competitor phase with similar modification

on request

Matrix
Sample(s)

each compound 1 mg/mL in eluent

Conditions

Eluent : acetonitrile - water (50:50, v/v) Flow rate: 0.56 mL/min Temperature: 25 °C Injection volume: 1.0 µL

Detection
UV, 254 nm
Note
The separation of pyridine and phenol is used to determine the base deactivation. On a good base deactivated phase pyridine is eluted well ahead of phenol. Furthermore the pyridine peak has to be symmetric. NUCLEODUR PolarTec shows a high resolution between pyridine and phenol, and the pyridine peak is symmetric. This indicates a very good base deactivation.
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2011
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
Dateien
Lädt...
Share via Mail
Print View