Selectivity comparison: Separation of nucleobases under completely aqueos conditions on NUCLEODUR PolarTec and a competitor phase with similar modification

Selectivity comparison: Separation of nucleobases under completely aqueos conditions on NUCLEODUR PolarTec and a competitor phase with similar modification

Selectivity comparison: Separation of nucleobases under completely aqueos conditions on NUCLEODUR PolarTec and a competitor phase with similar modification

Application Number
124672
Method
HPLC
Results

Legend:
1 - cytosine 2 - uracil 3 - adenine 4 - guanine 5 - thymine

Substances
cytosine; uracil; adenine; guanine; thymine
Product(s)
PhaseREFWebshop

NUCLEODUR PolarTec

760488.30

competitor phase with similar modification

Matrix
Sample(s)

each compound 1 mg/mL eluent

Conditions

Eluent : 30 mmol/L potassium dihydrogenphosphate, pH 3.0 Flow rate: 0.5 mL/min Temperature: 30 °C Injection volume: 1.0 µL

Detection
UV, 220 nm
Note
NUCLEODUR PolarTec phase shows under identical conditions a better separation of basic substances than a competitor phase with similar modification. Resolution and retention of the compounds are in all cases on NUCLEODUR PolarTec higher than on the competitor phase.
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2011
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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