Separation of flavonoids

Separation of flavonoids

Separation of flavonoids

Application Number
124650
Method
HPLC
Results

Legend:
1 - catechin 2 - epicatechin 3 - rutin 4 - fisetin 5 - naringenin 6 - quercetin 7 - isorhamnetin 8 - flavanone 9 - kaempferol

Substances
catechin; epicatechin; rutin; fisetin; naringenin; quercetin; isorhamnetin; flavanone; kaempferol
Product(s)
PhaseREFWebshop

NUCLEODUR PolarTec

760488.30

Matrix
Sample(s)

each compound 1 mg/mL in eluent

Conditions

Eluent A: acetonitrile with 0.1 % TFA Eluent B: water with 0.1 % TFA Gradient: 20 - 75 % A in 20 min Flow rate: 0.56 mL/min Temperature: 30 °C Injection volume: 2.5 µL

Detection
UV, 270 nm
Note
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2011
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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