Comparison in the separation of tricyclic antidepressants on NUCLEODUR PolarTec and a competitor phase with similar modification

Comparison in the separation of tricyclic antidepressants on NUCLEODUR PolarTec and a competitor phase with similar modification

Comparison in the separation of tricyclic antidepressants on NUCLEODUR PolarTec and a competitor phase with similar modification

Application Number
124622
Method
HPLC
Results

Legend:
1 - protryptiline 2 - doxepin 3 - imipramine 4 - amitryptiline 5 - trimipramine

Substances
protryptiline; doxepin; imipramine; amitryptiline; trimipramine
Product(s)
PhaseREFWebshop

NUCLEODUR PolarTec

760488.30

competitor phase with similar modification

Matrix
Sample(s)

each compound 1 mg/mL in eluent

Conditions

Eluent : methanol - 25 mmol/L KH2PO4, pH 7.0 (70:30, v/v) Flow rate: 0.66 mL/min Temperature: 30 °C Injection volume: 1.0 µL

Detection
UV, 254 nm
Note
Both phases show the protective mechanism of the 'embedded Group' for silanol interactions. A better end-capping of NUCLEODUR PolarTec, displays significantly better peak shapes for strong basic components like tricyclic antidepressants than the competitor phase.
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2011
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
Dateien
Lädt...
Share via Mail
Print View