Comparison of peak shape and retention times in the separation of tricyclic antidepressants on NUCLEODUR PolarTec and NUCLEODUR HTec

Comparison of peak shape and retention times in the separation of tricyclic antidepressants on NUCLEODUR PolarTec and NUCLEODUR HTec

Comparison of peak shape and retention times in the separation of tricyclic antidepressants on NUCLEODUR PolarTec and NUCLEODUR HTec

Application Number
124621
Method
HPLC
Results

Legend:
1 - protryptiline 2 - doxepin 3 - imipramine 4 - amitryptiline 5 - trimipramine

Substances
protryptiline; doxepin; imipramine; amitryptiline; trimipramine
Product(s)
PhaseREFWebshop

NUCLEODUR PolarTec

760488.30

NUCLEODUR C18 HTec

760315.30

Matrix
Sample(s)

each compound 1 mg/mL in eluent

Conditions

Eluent : methanol - 25 mmol/L KH2PO4, pH 7.0 (70:30, v/v) Flow rate: 0.66 mL/min Temperature: 30 °C Injection volume: 1.0 µL

Detection
UV, 254 nm
Note
NUCLEODUR PolarTec shows clearly more symmetrical peaks and shorter retention times for strongly basic components as opposed to NUCLEODUR C18 HTec. This phenomenon is based on the shielding mechanism of the embedded polar Group of NUCLEODUR PolarTec. Due to the polar group, the interaction between the analyte and the free silanol groups is prevented. .
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2011
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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