Eluent A: water
Eluent B: acetonitrile
Gradient: 0-100 % B in 25 min, hold for 5 min , 100-0 % B in 5 min
Flow rate: 0.2 mL/min
Temperature: 25 °C
Detection
MS, ESI positive
Needle voltage: 3 kV
Heater temperature: 400 °C
Cone voltage: 50 V
Mass range: 50 - 1000 m/z
Note
Bleeding of NUCLEODUR PolarTec is as good as bleeding of the competitor phase with similar modification.
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2011
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