Separation of purine alkaloids

Separation of purine alkaloids

Separation of purine alkaloids

Application Number
124580
Method
HPLC
Results

Legend:
1 - Theobromine 2 - Theophylline 3 - Caffeine

Substances
theobromine; theophylline; caffeine
Product(s)
PhaseREFWebshop

NUCLEODUR PolarTec

760488.30

Matrix
Sample(s)

each compound 1 mg/mL in eluent

Conditions

Eluent: acetonitrile - 5 mmol/L KH2PO4, pH 2 (5:95, v/v) Flow rate: 0.6 mL/min Temperature: 22 °C Injection volume : 5 µL

Detection
UV, 254 nm
Note
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2011
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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