Separation of water soluble vitamins on NUCLEODUR PolarTec

Separation of water soluble vitamins on NUCLEODUR PolarTec

Separation of water soluble vitamins on NUCLEODUR PolarTec

Application Number
124570
Method
HPLC
Results

Legend:
1 - vitamin B1 (thiamine) 2 - vitamin B6 (pyridoxine) 3 - vitamin B5 (pantothenic acid) 4 - vitamin BX (p-aminobenzoic acid) 5 - vitamin B2 (riboflavine)

Substances
vitamin B1 (thiamine); vitamin B6 (pyridoxine); vitamin B5 (pantothenic acid); vitamin BX (p-aminobenzoic acid); vitamin B2 (riboflavine)
Product(s)
PhaseREFWebshop

NUCLEODUR PolarTec

760488.30

Matrix
Sample(s)

each compound 1.0 mg/mL eluent

Conditions

Eluent: A) 25 mmol/L KH2PO4 , pH 3.0; B) acetonitrile Gradient: 10 % B for 3 min; 10 - 40 % B in 12 min Flow rate: 0.7 mL/min Temperature: 30 °C Injection volume : 5 µL

Detection
UV, 220 nm
Note
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2011
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
Dateien
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