Selectivity comparison: Separation of organic acids on NUCLEODUR PolarTec and a competitor phase with similar modification

Application Number: 124562
Method: HPLC
Results: Legend:
1 - dihydroxymandelic acid 2 - gallic acid 3 - dihydroxyphenylacetic acid 4 - 3,4-dihydroxybenzoic acid 5 - syringic acid 6 - vanillic acid 7 - 3-hydroxybenzoic acid 8 - 2,5-dihydroxybenzoic acid 9 - 2,4-dihydroxybenzoic acid
Substances: dihydroxymandelic acid; gallic acid; dihydroxyphenylacetic acid; 3,4-dihydroxybenzoic acid; syringic acid; vanillic acid; 3-hydroxybenzoic acid; 2,5-dihydroxybenzoic acid; 2,4-dihydroxybenzoic acid
Product(s): Phase REF Webshop
NUCLEODUR PolarTec

760488.30

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competitor phase with similar modification
Matrix
Sample(s): each compound 1 mg/mL eluent
Conditions: Eluent A: acetonitrile with 0.1 % TFA Eluent B: water with 0.1 % TFA Gradient: 20-60 % A in 12 min Flow rate: 0.73 mL/min Temperature: 20 °C Injection volume: 5.0 µL
Detection: UV, 254 nm
Note: Under identical conditions vanillic acid and 3-hydroxybenzoic acid are much better resolved on NUCLEODUR PolarTec phase than on the competitor phase. In addition, NUCLEODUR PolarTec has a significantly better retention of organic acids, which generally results in a better resolution of all components than on the competitive phase.
Author: HPLC department
Source: MACHEREY-NAGEL, Germany 2011
Disclaimer: Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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Phase	REF	Webshop
NUCLEODUR PolarTec	760488.30	Go to product details
competitor phase with similar modification

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