Selectivity comparison: Separation of organic acids on NUCLEODUR PolarTec and NUCLEODUR C18 HTec

Selectivity comparison: Separation of organic acids on NUCLEODUR PolarTec and NUCLEODUR C18 HTec

Selectivity comparison: Separation of organic acids on NUCLEODUR PolarTec and NUCLEODUR C18 HTec

Application Number
124561
Method
HPLC
Results

Legend:
1 - dihydroxymandelic acid 2 - gallic acid 3 - dihydroxyphenylacetic acid 4 - 3,4-dihydroxybenzoic acid 5 - syringic acid 6 - vanillic acid 7 - 3-hydroxybenzoic acid 8 - 2,5-dihydroxybenzoic acid 9 - 2,4-dihydroxybenzoic acid

Substances
dihydroxymandelic acid; gallic acid; dihydroxyphenylacetic acid; 3,4-dihydroxybenzoic acid; syringic acid; vanillic acid; 3-hydroxybenzoic acid; 2,5-dihydroxybenzoic acid; 2,4-dihydroxybenzoic acid
Product(s)
PhaseREFWebshop

NUCLEODUR PolarTec

760488.30

NUCLEODUR C18 HTec

760315.30

Matrix
Sample(s)

each compound 1 mg/mL eluent

Conditions

Eluent A: acetonitrile with 0.1 % TFA Eluent B: water with 0.1 % TFA Gradient: 20-60 % A in 12 min Flow rate: 0.73 mL/min Temperature: 20 °C Injection volume: 5.0 µL

Detection
UV, 254 nm
Note
NUCLEODUR PolarTec phase shows an improved selectivity for polar substances than a NUCLEODUR C18 HTec phase
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2011
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
Dateien
Lädt...
Share via Mail
Print View