Separation of Guanosine Phosphates

Separation of Guanosine Phosphates

Separation of Guanosine Phosphates

Application Number
123940
Method
HPLC
Results

Legend:
1 - guanosine monophosphate 2 - guanosine diphosphate 3 - guanosine triphosphate

Substances
guanosine monophosphate; guanosine diphosphate; guanosine triphosphate
Product(s)
PhaseREFWebshop

NUCLEODUR HILIC

760551.40

Matrix
Sample(s)

Standard samples: guanosine monophosphate: 0.5 mg/ml in eluent guanosine diphosphate: 0.5 mg/ml in eluent guanosine triphosphate: 0.5 mg/ml in eluent

Test sample: mixture from standard samples with ratio 1:2:2, v/v/v

Conditions

Eluent: 100 mM ammonium acetate, adjusted with acetic acid to pH 5.28 / acetonitrile (30:70, v/v) Flow rate: 1.3 mL/min Temperature: 25 °C Injection volume: 4 µL

Detection
UV, 259 nm
Note
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2010
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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