Separation of 18 PAHs on NUCLEODUR C18 PAH
Separation of 18 PAHs on NUCLEODUR C18 PAH
Separation of 18 PAHs on NUCLEODUR C18 PAH
Separation of 18 PAHs on NUCLEODUR C18 PAH
Separation of 18 PAHs on NUCLEODUR C18 PAH
- Application Number
- 123840
- Method
- HPLC
- Results
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Legend:
Chromatogram (blue) - UV detection Chromatogram (red) - Fluorescence detection 1 - naphthalene 2 - acenaphthylene (not detectable with fluorescence) 3 - 1-methylnaphthalene 4 - 2-methylnaphthalene 5 - acenaphthene 6 - fluorene 7 - phenanthrene 8 - anthracene 9 - fluoranthene 10 - pyrene 11 - benz[a]anthracene 12 - chrysene 13 - benzo[b]fluoranthene 14 - benzo[k]fluoranthene 15 - benzo[a]pyrene 16 - dibenz[ah]anthracene 17 - benzo[ghi]perylene 18 - indeno[1,2,3-cd]pyrene- Substances
- naphthalene; acenaphthylene; 1-methylnaphthalene; 2-methylnaphthalene; acenaphthene; fluorene; phenanthrene; anthracene; fluoranthene; pyrene; benz[a]anthracene; chrysene; benzo[b]fluoranthene; benzo[k]fluoranthene; benzo[a]pyrene; dibenz[ah]anthracene; benzo[ghi]perylene; indeno[1,2,3-cd]pyrene
- Product(s)
Phase REF Webshop NUCLEODUR C18 PAH
760784.40
- Matrix
- -
- Sample(s)
Concentrations: UV, 10 ng/µL Fluorescence, 10 ng/µL
- Conditions
- Eluent A: methanol / water (70:30, v/v)
- Eluent B: acetonitrile
- Gradient: 0-20 % B in 1.5 min, 20-50 % B in 1.5 min, 50-100 % B in 1.0 min, 100 % B for 3.0 min, 100-0 % B in 0.5 min
- Flow rate: 1.5 mL/min
- Temperature: 35 °C
Injection volumes:
- UV, 1 µL (10 ng per compound)
- Fluorescence, 0.5 µL (5 ng per compound)
- Detection
- UV, 254 nm Fluorescence, see chromatogram
- Note
- Author
- HPLC department
- Source
- MACHEREY-NAGEL, Germany 2010
- Disclaimer
- Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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