Determination of melamine in milk on NUCLEODUR HILIC with UV and LC-MS detection

Determination of melamine in milk on NUCLEODUR HILIC with UV and LC-MS detection

Determination of melamine in milk on NUCLEODUR HILIC with UV and LC-MS detection

Application Number
123090
Method
HPLC
Results
UV detection (100 ng/injection):

Title: UV detection (100 ng/injection):



Substances
melamine
Product(s)
PhaseREFWebshop

NUCLEODUR HILIC

760551.40

Matrix
milk
Sample(s)

Concentrations: 100 ng/µL and 100 pg/µL melamine in milk For a clean-up SPE Appl. No. 304920 is recommendable.

Conditions

Eluent: acetonitrile - 10 mmol ammonium formate, pH 4 (90:10, v/v) Flow rate: 0.6 mL/min Temperature: 25 °C Injection volume: 1.0 µL

Detection
UV, 210 nm LC-MS Mode: ESI+ Sample temp.: 400 °C Needle: 3.0 kV Cone voltage: 50 V
Note
Author
HPLC department
Source
MACHEREY-NAGEL, Germany 2009
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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