Determination of iso-α-Acids, α- and β-Acids in Isomerised Hop Pellets by HPLC

Determination of iso-α-Acids, α- and β-Acids in Isomerised Hop Pellets by HPLC

Determination of iso-α-Acids, α- and β-Acids in Isomerised Hop Pellets by HPLC

Application Number
121100
Method
HPLC
Results

Substances
isocohumulone; isohumulone; isoadhumulone; cohumulone; N/adhumulone; colupulone; N/adlupulone
Product(s)
PhaseREFWebshop

NUCLEODUR 100-5 C18 ec

760001.40

Matrix
hop
Sample(s)
Conditions

Eluent: A) methanol B) 750 ml methanol / 240 ml water / 10 ml ortho-phosphoric acid

Gradient: 100 % B for 9 min (270 nm) 100 % B for 8 min (314 nm) 100 - 65 % B in 8 min (314 nm) 65 - 100 % B in 5 min (314 nm)

Temperature: 35 °C

Flow rate: 1.0 mL/min

Detection
UV 270 nm / 314 nm
Note
Author
Biendl, M.; Virant, M.; Varju, P.
Source
Journal of the Institute of Brewing Vol. 110, No. 3, 2004, 242-243 European Brewery Convention
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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