Separation of Derivatised Amino Acids
Separation of Derivatised Amino Acids
Separation of Derivatised Amino Acids
- Application Number
- 120510
- Method
- HPLC
- Results
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Legend:
Asp aspartic acid; Glu glutamic acid; Asn asparagine; Ser serine; Gln glutamine; His histidine; Gly glycine; Thr threonine; Cit citrulline; Arg arginine; Ala alalanine; Tyr tyrosine; Val valine; Met methionine; Trp tryptophane; Phe phenylalanine; Ile isoleucine; Orn ornithine; Leu leucine; Lys lysine; Pro proline- Substances
- aspartic acid; glutamic acid; asparagine; serine; glutamine; histidine; glycine; threonine; citrulline; arginine; alanine; tyrosine; valine; methionine; tryptophan; phenylalanine; isoleucine; ornithine; leucine; lysine; proline
- Product(s)
Phase REF Webshop NUCLEODUR 100-5 C18 ec
760001.40
- Matrix
- Sample(s)
OPA derivatisation of amino acids and F-MOC derivatisation of proline
- Conditions
Eluent: A: methanol / acetonitrile (50:50 v/v); B: 40 mM sodium acetate buffer, pH 6.5 + 5% A gradient: 93% B for 5 min, 93 - 90% B in 2 min, 90 - 85% B in 8 min, 85 - 50% B in 7 min, 50 - 0% B in 3 min, 0% B for 3 min, 0 - 20% B in 1 min, 20 - 50% B in 1 min, 50 - 80% B in 1 min, 80 - 93% in 1 min. Flow rate: 0.8 mL/min for 3.1 min, then 1 mL/min Temperature: 35° C Inj. volume: 5 µL
- Detection
- Fluorescence, Ex 230 nm, Em 450 nm
- Note
- Author
- Wirtz
- Source
- Institut für Biochemie, Köln, Germany
- Disclaimer
- Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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