Quantification of the enantiomers of ofloxacin in biological fluids by HPLC
Quantification of the enantiomers of ofloxacin in biological fluids by HPLC
Quantification of the enantiomers of ofloxacin in biological fluids by HPLC
Quantification of the enantiomers of ofloxacin in biological fluids by HPLC
Quantification of the enantiomers of ofloxacin in biological fluids by HPLC
- Application Number
- 105830
- Method
- HPLC
- Results
.gif "see Note:")
Title: see Note:
.gif)
- Substances
- ofloxacin
- Product(s)
Phase REF Webshop RESOLVOSIL BSA-7
720046.40
RESOLVOSIL BSA-7
721402.30
NUCLEOSIL C18
720051.46
- Matrix
- serum; urine
- Sample(s)
Method A: without derivatization Method B: derivatization of ofloxacin with L-leucinamide via diphenylphosphinyl chloride activation
- Conditions
Method A Eluent: 0.2 mol/L phosphate buffer pH 8.0, 3 % 2-propanol Flow rate: 1.0 mL/min
Method B: Eluent: 0.2 mol/L phosphoric acid, adjusted to pH 1.85 with tetraethylammonium hydroxide solution - acetonitrile (80:20, v/v) Flow rate: 1.5 mL/min Temperature: 40 °C
- Detection
- Fluorescence, excitation 298 nm, emission 458 nm
- Note
- Method A: RESOLVOSIL BSA-7 column + guard column Method B: NUCLEOSIL C18 column Method B: Chromatogram of derivatized samples from a volunteer, serum taken 45 min after an intravenous dose of 50 ng of (±)-ofloxacin containing 0.176 µg/mL (-)-isomer and 0.166 µg/mL (+)-isomer, urine taken between 36 and 48h containing 0.912 µg/mL (-)-isomer and 0.540 µg/mL (+)-isomer.
- Author
- Lehr, K.-H. et al.
- Source
- J. Chromatogr. 425 (1988) 153 - 161
- Disclaimer
- Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
- Dateien
- Lädt...Lädt...