Chiral separation on achiral stationary phases with different functionalities using β-cyclodextrin in the mobile phase and application to bioanalysis and coupled columns

Chiral separation on achiral stationary phases with different functionalities using β-cyclodextrin in the mobile phase and application to bioanalysis and coupled columns

Chiral separation on achiral stationary phases with different functionalities using β-cyclodextrin in the mobile phase and application to bioanalysis and coupled columns

Application Number
105610
Method
HPLC
Results

Legend:
Chromatogram of racemic terbutaline, injected amount 200 pmol.

Substances
terbutaline
Product(s)
PhaseREFWebshop

NUCLEOSIL C18

720141.46

Matrix
Sample(s)
Conditions

Eluent: 8.4 mmol/L β-cyclodextrin in 0.05 mol/L ammonium acetate, adjusted to pH 6 with acetic acid Flow rate: 1 mL/min

Detection
amperometric, glassy carbon electrode at 0.9 V versus Ag/AgCl
Note
Author
Walhagen, A.; Edholm, L.-E.
Source
Chromatographia 32 (1991) 215 - 223
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