HPLC determination of chlordiazepoxide, its metabolites and oxaziridines generated after UV irradiation

Application Number: 104540
Method: HPLC
Results: Legend:
HPLC separation of chlordiazepoxide (4), 19 ng and its metabolites: demoxepam (1), 150 ng desmethyl chlordiazepoxide (2), 16 ng oxazepam (3), 480 ng desmethyldiazepam (5), 670 ng and its photoproducts after UV irradiation (70 min, < 10 °C): oxaziridine of desmethyl chlordiazepoxide (6) oxaziridine of demoxepam (7) oxaziridine of chlordiazepoxide (8)
Substances: chlordiazepoxide; demoxepam; desmethyl chlordiazepoxide; oxazepam; desmethyldiazepam; oxaziridines
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NUCLEOSIL C18

720141.46

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Matrix
Sample(s): UV irradiation for 70 min at < 10 °C
Conditions: Eluent: CH3CN - THF - 0.06 mol/L phosphate buffer, pH 5.8 (22:2:76, v/v/v) Temperature: 20 °C Flow rate: 1.0 mL/min
Detection: UV, 265 nm
Note
Author: Soentjens-Werts, V. et al.
Source: J. Chromatogr. A, 662 (1994) 255 - 262
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NUCLEOSIL C18	720141.46	Go to product details