Method for the determination of tryptophan in serum and CSF

Method for the determination of tryptophan in serum and CSF

Method for the determination of tryptophan in serum and CSF

Application Number
102190
Method
HPLC
Results

Legend:
Chromatograms: a) total serum Trp b) free serum Trp c) cerebrospinal fluid (CSF) Peaks: 1 - tryptophan (Trp) 2 - α-methyltryptophan (α-methyl-Trp)

Substances
tryptophan (Trp); α-methyltryptophan (α-methyl-Trp)
Product(s)
PhaseREFWebshop

NUCLEOSIL C18

720141.46

720120.46

Matrix
serum
Sample(s)
Conditions

Eluent: 10 mmol/L acetate solution pH 4.0 containing 14 % (v/v) methanol

Detection
Fluorescence: excitation 282 nm, emission 370 nm
Note
Author
Beck, O. et al.
Source
J. Chromatogr. 181 (1980) 100 - 102
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