Analysis of a mixture of 39 FAMEs included conjugated fatty acids with OPTIMA FFAPplus

Analysis of a mixture of 39 FAMEs included conjugated fatty acids with OPTIMA FFAPplus

Analysis of a mixture of 39 FAMEs included conjugated fatty acids with OPTIMA FFAPplus

Application Number
214880
Method
GC
Results
null

Legend: 1 - butyric acid methyl ester (C4:0) 2 - caproic acid methyl ester (C6:0) 3 - caprylic acid methyl ester (C8:0) 4 - capric acid methyl ester (C10:0) 5 - undecanoic acid methyl ester (C11:0) 6 - lauric acid methyl ester (C12:0) 7 - tridecanoic acid methyl ester (C13:0) 8 - myristic acid methyl ester (C14:0) 9 - myristoleic acid methyl ester (C14:1) 10 - pentadecanoic acid methyl ester (C15:0) 11 - cis-10-pentadecenoic acid methyl ester (C15:1) 12 - palmitic acid methyl ester (C16:0) 13 - hexadecenoic acid methyl ester (C16:1) 14 - margaric acid methyl ester (C17:0) 15 - cis-10-heptadecenoic acid (C17:1) 16 - stearic acid methyl ester (C18:0) 17 - oleic acid methyl ester (C18:1n9c) / elaidic acid methyl ester (C18:1n9t) 18 - linoleic acid methyl ester (C18:2n6c) / linolelaidic acid methyl ester (C18:2n6t) 19 - γ-linolenic acid methyl ester (C18:3n6) 20 - α-linolenic acid methyl ester (C18:3n3) 21 - cis-9,trans-11-octadecadienoic acid (9(Z),11(E)-conjugated linoleic acid, bovinic acid) 22 - trans-10,cis-12-octadecadienoic acid (10(E),12(Z)-conjugated linoleic acid) 23 - arachidic acid methyl ester (C20:0) 24 - cis-11-eicosenoic acid methyl ester (C20:1) 25 - cis-11,14-eicosadienoic acid methyl ester (C20:2) 26 - cis-8,11,14-eicosatrienoic acid methyl ester (C20:3n6) / heneicosanoic acid methyl ester (C21:0) 27 - arachidonic acid methyl ester (C20:4n6) 28 - cis-11,14,17-eicosatrienoic acid methyl ester (C20:3n3) 29 - cis-5,8,11,14,17-eicosapentaenoic acid methyl ester (C20:5n3) 30 - behenic acid methyl ester (C22:0) 31 - erucic acid methyl ester (C22:1n9) 32 - cis-13,16-docosadienoic acid methyl ester (C22:2) 33 - tricosanoic acid methyl ester (C23:0) 34 - lignoceric acid methyl ester (C24:0) 35 - nervonic acid methyl ester (C24:1) 36 - cis-4,7,10,13,16,19-docosahexaenoic acid (C22:6n3)

Substances
butyric acid methyl ester (C4:0); caproic acid methyl ester (C6:0); caprylic acid methyl ester (C8:0); capric acid methyl ester (C10:0); undecanoic acid methyl ester (C11:0); lauric acid methyl ester (C12:0); tridecanoic acid methyl ester (C13:0); myristic acid methyl ester (C14:0); myristoleic acid methyl ester (C14:1); pentadecanoic acid methyl ester (C15:0); cis-10-pentadecenoic acid methyl ester (C15:1); palmitic acid methyl ester (C16:0); hexadecenoic acid methyl ester (C16:1); margaric acid methyl ester (C17:0); cis-10-heptadecenoic acid (C17:1); stearic acid methyl ester (C18:0); oleic acid methyl ester (C18:1n9c); elaidic acid methyl ester (C18:1n9t); linoleic acid methyl ester (C18:2n6c); linolelaidic acid methyl ester (C18:2n6t); γ-linolenic acid methyl ester (C18:3n6); α-linolenic acid methyl ester (C18:3n3); cis-9,trans-11-octadecadienoic acid (9(Z),11(E)-conjugated linoleic acid, bovinic acid); trans-10,cis-12-octadecadienoic acid (10(E),12(Z)-conjugated linoleic acid); arachidic acid methyl ester (C20:0); cis-11-eicosenoic acid methyl ester (C20:1); cis-11,14-eicosadienoic acid methyl ester (C20:2); cis-8,11,14-eicosatrienoic acid methyl ester (C20:3n6); heneicosanoic acid; methyl ester (C21:0); arachidonic acid methyl ester (C20:4n6); cis-11,14,17-eicosatrienoic acid methyl ester (C20:3n3); cis-5,8,11,14,17-eicosapentaenoic acid methyl ester (C20:5n3); behenic acid methyl ester (C22:0); erucic acid methyl ester (C22:1n9); cis-13,16-docosadienoic acid methyl ester (C22:2); tricosanoic acid methyl ester (C23:0); lignoceric acid methyl ester (C24:0); nervonic acid methyl ester (C24:1); cis-4,7,10,13,16,19-docosahexaenoic acid (C22:6n3)
Product(s)
PhaseREFWebshop

OPTIMA FFAPplus

726241.60

Matrix
-
Sample(s)

Mixture of 39 FAMEs:

  • 37-Component FAME Mix (Supelco, item no. 18919)
  • 9(Z),11(E)-Conjugated Linoleic Acid (Cayman Chemical, item no. 90140)
  • 10(E),12(Z)-Conjugated Linoleic Acid (Cayman Chemical, item no. 90145)
Conditions
  • Injection volume: 1 µL, split 1:20
  • Injection temperature: 260 °C
  • Carrier gas: Helium, 1.2 mL/min
  • Temperature: 140 °C (2 min) --> 200 °C, 5 °C/min (15 min) --> 240 °C, 20 °C/min (25 min)
Detection
FID, 260 °C
Note
Customer used 50 m instead of 60 m. This has to be considered with regard to the retention time shown in the chromatogram.
Author
Most, E.
Source
Inst. f. Tierernährung & Ernährungphysiologie, Uni Gießen, Germany 2013
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.