Quantification of N-(3-chloro-2-hydroxypropyl)valine in human haemoglobin as a biomarker of epichlorohydrin exposure by gas chromatography-tandem mass spectrometry with stabl-isotope dilution

Quantification of N-(3-chloro-2-hydroxypropyl)valine in human haemoglobin as a biomarker of epichlorohydrin exposure by gas chromatography-tandem mass spectrometry with stabl-isotope dilution

Quantification of N-(3-chloro-2-hydroxypropyl)valine in human haemoglobin as a biomarker of epichlorohydrin exposure by gas chromatography-tandem mass spectrometry with stabl-isotope dilution

Application Number
213700
Method
GC
Results

Legend:
Partial GC-tandem MS chromatograms from the analysis of a sample from healthy subject not exposed to epichlorohydrin before (A) and after (B) addition of d0-CHPV. In both samples the concentration of the internal standard d5-CHPV was 40 pmol/g globin.

Substances
N-(3-chloro-2-hydroxypropyl)valine (CHPV)
Product(s)
PhaseREFWebshop

OPTIMA 1 MS

726205.30

Matrix
haemoglobin
Sample(s)

For preparation of toluene extract from blood samples see cited literature.

Conditions

Injection volume: 1 µL, splitless Injection temperature: PTV injector 120 °C --> 320 °C, 10 °C/min (4 min) Carrier gas: helium, 1.0 mL/min Temperature: 80 °C (1 min) --> 340 °C, 8 °C/min (2 min)

Detection
see Note:
Note
Detection: GC-tandem MS Transfer line temp.: 300 °C Ion source temp.: 180 °C Electron-capture negative-ion chemical ionisation (ECNICI) Reagent gas: methane, 530 Pa Electron energy: 100 eV Curent emission: 300 µA Collision-induced dissociation (CID) Collision gas: argon, 0.27 PA Collision energy: 15 eV Selected-reaction monitoring (SRM) product ion: m/z 301 precursor ions: m/z 422 for d0-CHPV, m/z 427 for d5-CHPV dwell time: 100 ms electron multiplier voltage: 2800 V
Author
Bader, M. et al.
Source
J. Chromatogr. B 877 (2009), 1402 - 1415
Disclaimer
Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL does not warrant the correctness of the applications.
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