Silylation with MSHFBA
Silylation with MSHFBA
Silylation with MSHFBA
Silylation with MSHFBA
Silylation with MSHFBA
- Application Number
- 213112
- Method
- GC
- Results
- Substances
- carboxylic acids; alcohols; phenols; amines; amino acids
- Product(s)
Phase REF Webshop MSHFBA
701260.110
- Matrix
- -
- Sample(s)
- Conditions
Dissolve 10-15 mg sample in 0.8 mL solvent, then add 0.2 mL of the silylation reagent. The reaction mixture can be heated to 60-70 °C for up to 1 hour and can be analyzed directly. If TFA is used as a solvent, proceed as follows [M. Donike, J. Chromatogr. 85 (1973) 1-7]: dissolve 1-2 mg sample in 100 µL TFA. Dropwise add 0.9 mL of the silylating reagent. After cooling the sample can be chromatographed directly.
- Detection
- -
- Note
- Recommended application: carboxylic acids, alcohols, phenols, primary and secondary amines and amino acids used either alone or in combination with a catalyst (TMCS, REF 701280.201, TSIM; REF 701310.201) or another silylation reagent with or without solvent. The by-product N-methylheptafluorobutyric amide has a lower retention time than the silylating reagent. Especially useful for flame ionisation detection due to the large ratio of fluorine to silicon of 7:1, since degradation of the excess of MSHFBA does not produce SiO2 but volatile, non-corrosive silicon compounds.
- Author
- MACHEREY-NAGEL
- Source
- GC department (2007)
- Disclaimer
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